E2F-4 (A-20): sc-1082. Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.
E2F-4 (A-20): sc-1082. Western blot analysis of E2F-4 expression in Jurkat (A) and Y79 (B) nuclear extracts.
ChIP analysis of c-Myc promoter occupancy in response to lithium stimulation in serum synchronized C2C12 cells. Antibodies tested include HDAC1 (H-15): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (A), β-catenin (H-120): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (B), E2F-4 (A-20): sc-1082, E2F-4 (D-3): sc-6851, E2F-4 (RK-13): sc-511 and E2F-4 (C-20): sc-866 (C), and p130 (C-20): sc-317, p130 (211.6): sc-9963 and p130 (H-125): sc-20678 (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.
E2F-4 (A-20): sc-1082. Western blot analysis of E2F-4 expression in non-transfected: sc-117752 (A) and mouse E2F-4 transfected: sc-119883 (B) 293T whole cell lysates.
E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated chicken anti-rabbit IgG: sc-2990. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated donkey anti-rabbit IgG: sc-2090. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by PE-conjugated mouse anti-rabbit IgG: sc-3753. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
goat anti-rabbit IgG-PE: sc-3739. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by PE-conjugated goat anti-rabbit IgG: sc-3739. Black line histogram represents the isotype control, normal rabbit IgG:sc-3888. Antibody tested:E2F-4 (A-20): sc-1082.