Anti-P2RX7 antibody
| Name | Anti-P2RX7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab77413 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide from the extracellular domain of mouse P2RX7, conjugated to an immunogenic carrier protein |
| Blocking Peptide | P2RX7 peptide |
| Description | Rabbit Polyclonal |
| Gene | P2RX7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab77413 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77413, used at a 1/100 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-P2RX7 antibody (ab77413) at 1/250 dilutionLane 1 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell LysateLane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.