Western blot analysis of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody (C-term) (Cat# 102-16696). 102-16696 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot analysis of Sirt3 Antibody (C-term) (Cat# 102-16696) in mouse brain tissue lysates (35ug/lane).Sirt3 (arrow) was detected using the purified Pab.
Western blot analysis of Sirt3 Antibody (C-term) (Cat# 102-16696) in CHO cell line lysates (35ug/lane).Sirt3 (arrow) was detected using the purified Pab.
Sirt3 Antibody (C-term) (Cat# 102-16696) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.