Anti-p35 antibody
| Name | Anti-p35 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66064 |
| Prices | $387.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | ELISA WB IHC-P |
| Species Reactivities | Mouse, Human, Rat, Horse, Bovine, Dog, Pig, Chimpanzee, Monkey, Opossum |
| Antigen | Recombinant tagged fragment: CRDVISSEVG SDHELQAVLL TCLYLSYSYM GNEISYPLKP FLVESCKEAF WDRCLSVINL MSSKMLQINA DPHYFTQVFS DLKNESGQED KKRLLLGLDR, corresponding to amino acids 208-308 of Human p35 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | CDK5R1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-p35 antibody (ab66064) at 1 µg/mlLane 1 : p35 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
IHC image of ab66064 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66064, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.