Western blot analysis of extracts from MUTZ-3 cells, undifferentiated (-) or differentiated (+) into Langerhans-like cells by treatment with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Transforming Growth Factor β1 (hTGF-β1) #8915 (10 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 10 d, using Langerin (D9H7R) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Langerin protein (hLangerin-Myc/DDK; +), using Langerin (D9H7R) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of MUTZ-3 cells, undifferentiated (left) or differentiated (right) into Langerhans-like cells by treatment with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Transforming Growth Factor β1 (hTGF-β1) #8915 (10 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 10 d (right), using Langerin (D9H7R) XP ® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of MUTZ-3 cells, undifferentiated (blue) or differentiated (green) into Langerhans-like cells by treatment with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Transforming Growth Factor β1 (hTGF-β1) #8915 (10 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 10 d, using Langerin (D9H7R) XP ® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.