Anti-p400 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab70301
• Prices: $398.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP ChIP ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Rat, Horse, Guinea Pig, Bovine, Pig, Chimpanzee, Monkey, Ape, Hamster, Orangutan, Elephant
• Antigen: Synthetic peptide corresponding to a region between C terminal residues 3075 and 3124 of human p400
• Description: Rabbit Polyclonal
• Gene: EP400
• Conjugate: Unconjugated

Product images

All lanes : Anti-p400 antibody - ChIP Grade (ab70301) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg

Detection of Human p400 by Western Blot of Immunprecipitate. Lane 1: Irrelevant antibody used for precipitation. Lane 2: ab70301 at 0.04µg/ml staining p400 in HeLa whole cell lysates immunoprecipitated using ab70301 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 3: Control IgG used for precipitation. Detection: Chemiluminescence with exposure time 10 seconds.

ab70301 (1/200) staining p400 in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized using 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview

ICC/IF image of ab70301 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70301, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z. - Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z.

Bellucci L, Dalvai M, Kocanova S, Moutahir F, Bystricky K. PLoS One. 2013;8(1):e54102.

The E1A-associated p400 protein modulates cell fate decisions by the regulation - The E1A-associated p400 protein modulates cell fate decisions by the regulation

Mattera L, Courilleau C, Legube G, Ueda T, Fukunaga R, Chevillard-Briet M, Canitrot Y, Escaffit F, Trouche D. PLoS Genet. 2010 Jun 10;6(6):e1000983.

A Myc network accounts for similarities between embryonic stem and cancer cell - A Myc network accounts for similarities between embryonic stem and cancer cell

Kim J, Woo AJ, Chu J, Snow JW, Fujiwara Y, Kim CG, Cantor AB, Orkin SH. Cell. 2010 Oct 15;143(2):313-24.

VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400. - VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.

Young AP, Schlisio S, Minamishima YA, Zhang Q, Li L, Grisanzio C, Signoretti S, Kaelin WG Jr. Nat Cell Biol. 2008 Mar;10(3):361-9.