Anti-P4HB antibody (ab70415) at 1/500 dilution + Human liver tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
All lanes : Anti-P4HB antibody (ab70415) at 1/500 dilutionLane 1 : P4HB transfected 293T lysate Lane 2 : Non-transfected 293T lysate Lysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
ab70415 at 10µg/ml staining P4HB in Hela cells by Immunocytochemistry/Immunofluorescence.
IHC image of ab70415 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70415, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.