P4HB

Gene Symbol P4HB
Entrez Gene 5034
Alt Symbol CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB
Species Human
Gene Type protein-coding
Description prolyl 4-hydroxylase, beta polypeptide
Other Description cellular thyroid hormone-binding protein|collagen prolyl 4-hydroxylase beta|glutathione-insulin transhydrogenase|p55|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide|prolyl 4-hydroxylase subunit beta|protein disulfide isomerase family A, member 1|protein disulfide isomerase-associated 1|protein disulfide isomerase/oxidoreductase|protein disulfide-isomerase|protocollagen hydroxylase|thyroid hormone-binding protein p55
Swissprots P30037 P32079 Q6LDE5 B2RDQ2 P07237 Q15205
Accessions AAB22262 AAC13652 CCQ43001 CDM55388 CDM55569 EAW89690 EAW89691 EAW89692 EAW89693 EAW89694 EAW89695 EAW89696 P07237 AB062434 BAB93497 AK075503 AK095938 BAG53171 AK128783 BAG54732 AK296206 BAG58932 AK297081 BAG59598 AK297967 BAG60277 AK298005 BAG60311 AK300561 BAG62267 AK308600 AK315631 BAG37999 AU131354 BC010859 AAH10859 BC014504 AAH14504 BC018801 BC029617 AAH29617 BC071892 AAH71892 BX353203 CA412209 DB466371 J02783 AAA61169 JF432204 ADZ15421 X05130 CAA28775 X07077 CAA30112 NM_000918 NP_000909
Function This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. {ECO:0000269|PubMed:10636893, ECO:0000269|PubMed:12485997}.
Subcellular Location Endoplasmic reticulum lumen. Melanosome. Cell membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Note=Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. {ECO:0000305}.
Top Pathways Protein processing in endoplasmic reticulum

PDI (C81H6) Rabbit mAb (Alexa Fluor ® 594 Conjugate) - 8615 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C81H6
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red) and β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 488 Conjugate) #8844 (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

PDI (C81H6) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 5051 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C81H6
Immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green) and a β-Actin Mouse mAb (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

PDI (C81H6) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 8463 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone C81H6

PDI (C81H6) Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 8448 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone C81H6

PDI Antibody - 2446 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using PDI Antibody.

PDI (C81H6) Rabbit mAb - 3501 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C81H6
Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb.

PDI (A-1) - sc-376370 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PDI (A-1): sc-376370. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells.

PDI (C-2) - sc-74551 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PDI (C-2): sc-74551. Western blot analysis of PDI expression in COLO 320DM (A), Hep G2 (B), A-431 (C), TT (D) and c4 (E) whole cell lysates and mouse liver (F) and rat liver (G) tissue extracts.

PDI (C-5) - sc-376369 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PDI (C-5): sc-376369. Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic staining of glandular cells.

PDI (E-20) - sc-17222 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDI (E-20): sc-17222. Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.

PDI (F-11) - sc-166474 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PDI (F-11): sc-166474. Western blot analysis of PDI expression in non-transfected: sc-110760 (A) and human PDI transfected: sc-111237 (B) 293 whole cell lysates.

PDI (G-20) - sc-30931 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDI (G-20): sc-30931. Western blot analysis of PDI expression in non-transfected: sc-117752 (A) and human PDI transfected: sc-111237 (B) 293T whole cell lysates.

PDI (H-160) - sc-20132 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PDI (H-160): sc-20132. Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.

PDI (H-17) - sc-30932 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PDI (H-17): sc-30932. Western blot analysis of PDI expression in non-transfected: sc-110760 (A) and human PDI transfected: sc-111237 (B) 293 whole cell lysates.

PDI (34) - sc-136230 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PDI (34): sc-136230. Western blot analysis of PDI expression in HCT-8 whole cell lysate.

Anti-P4HB antibody - ab85564 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab85564 at a 1/50 dilution staining P4HB in human small intestine tissue by IHC-P.

Anti-P4HB antibody - ab70415 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-P4HB antibody (ab70415) at 1/500 dilution + Human liver tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-P4HB antibody [EPR9498] - ab137119 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9498
All lanes : Anti-P4HB antibody [EPR9498] (ab137119) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : A431 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-P4HB antibody [EPR9499] - ab137110 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9499
All lanes : Anti-P4HB antibody [EPR9499] (ab137110) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP-labeled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique

Anti-P4HB antibody [EPR9499] (Alexa Fluor® 488) - ab202820 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9499
ab202820 staining P4HB in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202820 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-P4HB antibody [EPR9499] (Alexa Fluor® 647) - ab202821 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9499
ab202821 staining P4HB in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202821 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-P4HB antibody - C-terminal - ab135531 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-P4HB antibody - C-terminal (ab135531) at 1/50 dilutionLane 1 : CEM cell line lysateLane 2 : HepG2 cell line lysateLane 3 : A2058 cell line lysateLysates/proteins at 35 µg per lane.

Anti-PDI / P4HB Antibody - LS-C38112 from LifeSpan Bioscience

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PDI / P4HB Antibody - LS-C38116 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-PDI / P4HB Antibody (clone 6-9H6) - LS-C96414 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6-9H6
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