All lanes : Anti-P4HB antibody - C-terminal (ab135531) at 1/50 dilutionLane 1 : CEM cell line lysateLane 2 : HepG2 cell line lysateLane 3 : A2058 cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labelling P4HB with ab135531 at 1/50 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells labelling P4HB with ab135531 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.