Anti-p53 (acetyl K373) antibody [EP356(2)AY]
| Name | Anti-p53 (acetyl K373) antibody [EP356(2)AY] |
|---|---|
| Supplier | Abcam |
| Catalog | ab62376 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP356(2)AY |
| Applications | WB IHC-P ICC/IF FC |
| Species Reactivities | Human |
| Antigen | A synthetic acetylated peptide corresponding to residues surrounding Ly373 of human p53 |
| Blocking Peptide | p53 (acetyl K373) peptide |
| Description | Rabbit Monoclonal |
| Gene | TP53 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376) at 1/100000 dilutionLane 1 : HepG2 cell lysate untreatedLane 2 : HepG2 cell lysate treated with etoposide + trichostatin ALysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5713_ab62376wb.gif)
All lanes : Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376) at 1/100000 dilutionLane 1 : HepG2 cell lysate untreatedLane 2 : HepG2 cell lysate treated with etoposide + trichostatin ALysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution
ab62376 at 1/100 dilution staining p53 in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Overlay histogram showing HeLa cells stained with ab62376 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62376, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 - Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1
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S100A6 competes with the TAZ2 domain of p300 for binding to p53 and attenuates - S100A6 competes with the TAZ2 domain of p300 for binding to p53 and attenuates
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Regulation of SIRT1 activity by genotoxic stress. - Regulation of SIRT1 activity by genotoxic stress.
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Basal dynamics of p53 reveal transcriptionally attenuated pulses in cycling - Basal dynamics of p53 reveal transcriptionally attenuated pulses in cycling
Loewer A, Batchelor E, Gaglia G, Lahav G. Cell. 2010 Jul 9;142(1):89-100.
Identification of ANKRD11 as a p53 coactivator. - Identification of ANKRD11 as a p53 coactivator.
Neilsen PM, Cheney KM, Li CW, Chen JD, Cawrse JE, Schulz RB, Powell JA, Kumar R, Callen DF. J Cell Sci. 2008 Nov 1;121(Pt 21):3541-52.