Anti-p53 (acetyl K382) antibody

• Supplier: Abcam
• Catalog: ab37318
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human p53
• Blocking Peptide: Human p53 (acetyl K382) peptide
• Description: Rabbit Polyclonal
• Gene: TP53
• Conjugate: Unconjugated

Product images

All lanes : Anti-p53 (acetyl K382) antibody (ab37318) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : Caco2 (Human colonic carcinoma cell line) Whole Cell LysateLane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with Human p53 (acetyl K382) peptide (ab37536) at 1 µg/mlLane 4 : Caco2 (Human colonic carcinoma cell line) Whole Cell Lysate with Human p53 (acetyl K382) peptide (ab37536) at 1 µg/mlLane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate (ab28419) with Human p53 peptide (ab42656) at 1 µg/mlLane 6 : Caco2 (Human colonic carcinoma cell line) Whole Cell Lysate with Human p53 peptide (ab42656) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-p53 (acetyl K382) antibody (ab37318) at 1/250 dilutionLane 1 : Whole cell lysate prepared from Human Caco-2 epithelial colorectal adenocarcinoma cellsLane 2 : Whole cell lysate prepared from Human Caco-2 epithelial colorectal adenocarcinoma cellsLane 3 : Whole cell lysate prepared from Human Caco-2 epithelial colorectal adenocarcinoma cellsLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG conjugated to HRP at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab37318 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37318, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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