Anti-p53 (phospho S315) antibody
| Name | Anti-p53 (phospho S315) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab1647 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide derived from within residues 300 to the C-terminus of Human p53 |
| Blocking Peptide | Human p53 (phospho S315) peptide |
| Description | Rabbit Polyclonal |
| Gene | TP53 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
The image shows staining of neoplastic cells in breast carcinoma tissue. Antigen retrieval was performed using Tris/EDTA at pH9.
ICC/IF image of ab1647 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 10 min) and incubated with the antibody (ab1647, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red). The exact cellular location of p53 (phospho S315) is not currently well described so we cannot say for certain if the localisation of the primary in this analysis is correct. However, the control experiment using only the secondary antibody gave no signal in the Alexa Fluor® 488 channel so the signal seen in the image above is specific to ab1647.
All lanes : Anti-p53 (phospho S315) antibody (ab1647) at 1 µg/mlLane 1 : Human Embryonic Stem Cell LysateLane 2 : Hela lysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 4 : Human Embryonic Stem Cell Lysate with Human p53 (phospho S315) peptide (ab28896) at 1 µg/mlLane 5 : Hela lysate with Human p53 (phospho S315) peptide (ab28896) at 1 µg/mlLane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) with Human p53 (phospho S315) peptide (ab28896) at 1 µg/mlLane 7 : Human Embryonic Stem Cell Lysate with p53 (unmodified ) peptide (ab28897) at 1 µg/mlLane 8 : Hela lysate with p53 (unmodified ) peptide (ab28897) at 1 µg/mlLane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) with p53 (unmodified ) peptide (ab28897) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutionPerformed under reducing conditions.
Product References
Diabetes-induced oxidative dna damage alters p53-p21CIP1/Waf1 signaling in the - Diabetes-induced oxidative dna damage alters p53-p21CIP1/Waf1 signaling in the
Kilarkaje N, Al-Bader MM. Reprod Sci. 2015 Jan;22(1):102-12.
Choice of fixative is crucial to successful immunohistochemical detection of - Choice of fixative is crucial to successful immunohistochemical detection of
Burns JA, Li Y, Cheney CA, Ou Y, Franlin-Pfeifer LL, Kuklin N, Zhang ZQ. J Histochem Cytochem. 2009 Mar;57(3):257-64.