Anti-p53R2 antibody
| Name | Anti-p53R2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab8105 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: GDPERPEAAGLDQDER , corresponding to amino acids 2-17 of Human p53R2 |
| Description | Rabbit Polyclonal |
| Gene | RRM2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-p53R2 antibody (ab8105) at 1/1000 dilution + U2OS whole cell lysate treated with UV irradiation.SecondaryGoat anti-rabbit at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-p53R2 antibody (ab8105) at 1 µg/mlLane 1 : A431 cell lysateLane 2 : 3T3 cell lysate with absence of blocking peptideLane 3 : 3T3 cell lysate with presence of blocking peptide
ab8105 at 1µg/ml staining p53R2 in lung tissue by IHC
ICC/IF image of ab8105 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8105, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of p53R2 in Human Lung cells using ab8105 at 20 ug/ml.
Product References
Breaking the invulnerability of cancer stem cells: two-step strategy to kill the - Breaking the invulnerability of cancer stem cells: two-step strategy to kill the
Morgenroth A, Vogg AT, Zlatopolskiy BD, Siluschek M, Oedekoven C, Mottaghy FM. Mol Cancer Ther. 2014 Jan;13(1):144-53.
p21-mediated RNR2 repression restricts HIV-1 replication in macrophages by - p21-mediated RNR2 repression restricts HIV-1 replication in macrophages by
Allouch A, David A, Amie SM, Lahouassa H, Chartier L, Margottin-Goguet F, Barre-Sinoussi F, Kim B, Saez-Cirion A, Pancino G. Proc Natl Acad Sci U S A. 2013 Oct 15;110(42):E3997-4006. doi:
Ribonucleotide reductase inhibition restores platinum-sensitivity in - Ribonucleotide reductase inhibition restores platinum-sensitivity in
Kunos C, Radivoyevitch T, Abdul-Karim FW, Fanning J, Abulafia O, Bonebrake AJ, Usha L. J Transl Med. 2012 Apr 27;10:79.
Deoxyribonucleotide metabolism in cycling and resting human fibroblasts with a - Deoxyribonucleotide metabolism in cycling and resting human fibroblasts with a
Pontarin G, Ferraro P, Rampazzo C, Kollberg G, Holme E, Reichard P, Bianchi V. J Biol Chem. 2011 Apr 1;286(13):11132-40.
Kearns-Sayre syndrome caused by defective R1/p53R2 assembly. - Kearns-Sayre syndrome caused by defective R1/p53R2 assembly.
Pitceathly RD, Fassone E, Taanman JW, Sadowski M, Fratter C, Mudanohwo EE, Woodward CE, Sweeney MG, Holton JL, Hanna MG, Rahman S. J Med Genet. 2011 Sep;48(9):610-7.
A heterozygous truncating mutation in RRM2B causes autosomal-dominant progressive - A heterozygous truncating mutation in RRM2B causes autosomal-dominant progressive
Tyynismaa H, Ylikallio E, Patel M, Molnar MJ, Haller RG, Suomalainen A. Am J Hum Genet. 2009 Aug;85(2):290-5.
p53 improves aerobic exercise capacity and augments skeletal muscle mitochondrial - p53 improves aerobic exercise capacity and augments skeletal muscle mitochondrial
Park JY, Wang PY, Matsumoto T, Sung HJ, Ma W, Choi JW, Anderson SA, Leary SC, Balaban RS, Kang JG, Hwang PM. Circ Res. 2009 Sep 25;105(7):705-12, 11 p following 712. doi:
Ribonucleotide reduction is a cytosolic process in mammalian cells independently - Ribonucleotide reduction is a cytosolic process in mammalian cells independently
Pontarin G, Fijolek A, Pizzo P, Ferraro P, Rampazzo C, Pozzan T, Thelander L, Reichard PA, Bianchi V. Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17801-6. doi:
Mutation of RRM2B, encoding p53-controlled ribonucleotide reductase (p53R2), - Mutation of RRM2B, encoding p53-controlled ribonucleotide reductase (p53R2),
Bourdon A, Minai L, Serre V, Jais JP, Sarzi E, Aubert S, Chretien D, de Lonlay P, Paquis-Flucklinger V, Arakawa H, Nakamura Y, Munnich A, Rotig A. Nat Genet. 2007 Jun;39(6):776-80. Epub 2007 May 7.
p53R2-dependent ribonucleotide reduction provides deoxyribonucleotides in - p53R2-dependent ribonucleotide reduction provides deoxyribonucleotides in
Pontarin G, Ferraro P, Hakansson P, Thelander L, Reichard P, Bianchi V. J Biol Chem. 2007 Jun 8;282(23):16820-8. Epub 2007 Apr 7.