RRM2B

Gene Symbol RRM2B
Entrez Gene 50484
Alt Symbol MTDPS8A, MTDPS8B, P53R2
Species Human
Gene Type protein-coding
Description ribonucleotide reductase M2 B (TP53 inducible)
Other Description TP53-inducible ribonucleotide reductase M2 B|p53-inducible ribonucleotide reductase small subunit 2 homolog|p53-inducible ribonucleotide reductase small subunit 2 short form beta|p53-inducible ribonucleotide reductase small subunit 2-like protein|ribonucleoside-diphosphate reductase subunit M2 B
Swissprots Q86YE3 B4E2N4 Q75PQ6 Q17R22 Q75PY9 Q9NUW3 Q75PQ7 Q9NTD8 Q9NPD6 Q75PY8 Q7LG56
Accessions AAY29059 BAA92493 EAW91840 EAW91841 EAW91842 Q7LG56 AB036063 BAA92434 AB163437 BAD11774 AB163438 BAD11775 AB166669 BAD12265 AB166670 BAD12266 AB166671 BAD12267 AK001965 BAA92005 AK023605 AK304354 BAG65196 AL137348 CAB70703 BC042468 AAH42468 BC042948 BC108261 AAI08262 BC117496 AAI17497 BC130628 AAI30629 BX509409 CR627376 CAH10473 DC308409 EU831630 ACE87230 EU831715 ACE86549 HQ258190 ADR82944 NM_001172477 NP_001165948 NM_001172478 NP_001165949 NM_015713 NP_056528
Function Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. {ECO:0000269|PubMed:10716435, ECO:0000269|PubMed:11517226, ECO:0000269|PubMed:11719458}.
Subcellular Location Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus in response to DNA damage.
Tissue Specificity Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. {ECO:0000269|PubMed:14583450}.
Top Pathways p53 signaling pathway, Purine metabolism, Glutathione metabolism, Pyrimidine metabolism

p53R2 (B-10) - sc-137175 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p53R2 (B-10): sc-137175. Western blot analysis of p53R2 If expression in non-transfected: sc-117752 (A) and human p53R2 If transfected: sc-111702 (B) 293T whole cell lysates.

p53R2 (H-300) - sc-98384 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p53R2 (H-300): sc-98384. Western blot analysis of p53R2 expression in SW480 (A) and MCF7 (B) whole cell lysates.

p53R2 (A-5) - sc-137174 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p53R2 (A-5): sc-137174. Western blot analysis of p53R2 If expression in non-transfected: sc-117752 (A) and human p53R2 If transfected: sc-111702 (B) 293T whole cell lysates.

p53R2 (H-3) - sc-137173 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p53R2 (H-3): sc-137173. Western blot analysis of p53R2 If expression in non-transfected: sc-117752 (A) and human p53R2 If transfected: sc-111702 (B) 293T whole cell lysates.

p53R2 (N-16) - sc-10840 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p53R2 (N-16): sc-10840. Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic and nuclear staining of glandular cells.

Anti-p53R2 antibody - ab8105 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-p53R2 antibody (ab8105) at 1/1000 dilution + U2OS whole cell lysate treated with UV irradiation.SecondaryGoat anti-rabbit at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-p53R2 antibody - ab63814 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p53R2 antibody (ab63814) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 3 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-p53R2 antibody - ab130321 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-p53R2 antibody (ab130321) at 1/100 dilution + HepG2 cell lysate at 35 µg

Anti-p53R2 antibody [EPR8816] - ab154194 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8816
All lanes : Anti-p53R2 antibody [EPR8816] (ab154194) at 1/1000 dilutionLane 1 : Human fetal muscle lysateLane 2 : MCF7 cell lysateLane 3 : SW480 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-p53R2 antibody - C-terminal - ab191055 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p53R2 antibody - C-terminal (ab191055) at 0.5 µg/mlLane 1 : Rat thymus tissue lysateLane 2 : MCF7 cell lysateLane 3 : A431 cell lysateLane 4 : HeLa cell lysate

Anti-RRM2B / P53R2 Antibody (aa2-17) IHC-plus™ - LS-B545 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RRM2B / p53R2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B545 concentration 5 ug/ml.

Anti-RRM2B / P53R2 Antibody - LS-C153107 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RRM2B Antibody (N-Terminus) - LS-C189144 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RRM2B Antibody (Phe156) - LS-C193220 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RRM2B / P53R2 Antibody (aa128-157, AP) - LS-C268615 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRM2B / P53R2 Antibody (aa128-157, APC) - LS-C268616 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRM2B / P53R2 Antibody (aa128-157, Biotin) - LS-C268617 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRM2B / P53R2 Antibody (aa128-157, FITC) - LS-C268618 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRM2B / P53R2 Antibody (aa128-157, Azide-free, HRP) - LS-C268619 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRM2B / P53R2 Antibody (aa128-157, PE) - LS-C268622 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRM2B / P53R2 Antibody (aa127-157) - LS-C324839 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RRM2B / P53R2 Antibody (aa128-157) - LS-C162769 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RRM2B Antibody western blot of HepG2 cell line lysates (35 ug/lane). The RRM2B antibody detected the RRM2B protein (arrow).

Anti-RRM2B / P53R2 Antibody (aa81-130) - LS-C201502 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human 293T. RRM2B antibody dilution 1.0 ug/ml.

Anti-RRM2B / P53R2 Antibody (N-Terminus) - LS-C154254 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-RRM2B antibody - Western Blot. Western blot of affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4:293T transfected with ScRNA. Lane 5:293T transfected with p53R2 SiRNA The band at 40.7 kD, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kD. The highest band at 44.3 kD corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4C. ECL detection was used.

Anti-RRM2B / P53R2 Antibody (aa318-332) - LS-C313179 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB of RRM2B antibody. Lane 1: Rat Thymus Tissue Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: A431 Cell Lysate. Lane 4: HELA Cell Lysate..
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