Anti-p63 antibody [EPR5701]

• Supplier: Abcam
• Catalog: ab124762
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5701
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Rabbit, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p63 aa 1-250
• Description: Rabbit Monoclonal
• Gene: TP63
• Conjugate: Unconjugated

Product images

Immunofluorescent staining of A431 cells (fixed with 4% PFA and permeablized with TritonX 100) with purified ab124762 at a dilution of 1/60. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

Anti-p63 antibody [EPR5701] (ab124762) at 1/1200 dilution (purified) + MDA-MB-435 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human prostate with purified ab124762 at a working dilution of 1 in 12000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Unpurified ab124762 staining p63 in Human corneal limbal epithelial cells (primary culture) by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with methanol and permeabilized with 0.3% Triton X-100 for 5 minutes. Samples were incubated with primary antibody (1/100 in PBS + 10% Goat serum) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Immunofluorescent staining of A431 cells (fixed with 4% PFA and permeablized with TritonX 100) with unpurified ab124762 at a dilution of 1/60. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

Overlay histogram showing A431 cells stained with unpurified ab124762 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab124762, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-p63 antibody [EPR5701] (ab124762) at 1/1000 dilution (unpurified)Lane 1 : Human bladder lysateLane 2 : Human kidney lysateLane 3 : Human skin lysateLane 4 : Human tonsil lysateLane 5 : PC 3 cell lysateLane 6 : MDA-MB-435 cell lysateLane 7 : PC12 cell lysateLane 8 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Immunohistochemical staining of p63 in paraffin-embedded human cervical carcinoma tissue with unpurified ab124762, at a 1/500 dilution.

Unpurified ab124762 showing positive staining in prostate hyperplasia tissue.

Unpurified ab124762 showing positive staining in normal tonsil tissue.

Unpurified ab124762 showing negative staining in skeletal muscle tissue.

Product References

Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor - Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor

Yu XM, Li CW, Chao SS, Li YY, Yan Y, Zhao XN, Yu FG, Liu J, Shen L, Pan XL, Shi L, Wang de Y. Sci Rep. 2014 Apr 9;4:4619.

Respiratory syncytial virus can infect basal cells and alter human airway - Respiratory syncytial virus can infect basal cells and alter human airway

Persson BD, Jaffe AB, Fearns R, Danahay H. PLoS One. 2014 Jul 17;9(7):e102368.

S100A2 promoter-driven conditionally replicative adenovirus targets - S100A2 promoter-driven conditionally replicative adenovirus targets

Lee K, Yun ST, Yun CO, Ahn BY, Jo EC. Gene Ther. 2012 Oct;19(10):967-77.