Anti-p63 antibody [Y289]

• Supplier: Abcam
• Catalog: ab32353
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y289
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p63 aa 650 to the C-terminus (C terminal)
• Description: Rabbit Monoclonal
• Gene: TP63
• Conjugate: Unconjugated

Product images

Anti-p63 antibody [Y289] (ab32353) at 1/4000 dilution (unpurified) + A431 cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-p63 antibody [Y289] (ab32353) at 1/10000 dilution (purified) + A431 cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular skin tissue labelling p63 with purified ab32353 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunocytochemsitry/Immunofluorescence analysis of A431 cells labelling p63 (red) with purified ab32353 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunofluorescent staining of A431 cells using ab32353 at 1/250 dilution.