Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta using ab184551 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (green). Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution
Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta using ab184551 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 488) secondary at a 1/200 dilution (green) costained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded Human pancreas labeling P70 S6 Kinase beta using ab184551 at a 1/50 dilution, counterstained with Hematoxylin.
All lanes : Anti-P70 S6 Kinase beta antibody [EPR14956] - N-terminal (ab184551) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-P70 S6 Kinase beta antibody [EPR14956] - N-terminal (ab184551) at 1/10000 dilution + Human fetal liver lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution