Anti-p75 NGF Receptor antibody
| Name | Anti-p75 NGF Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab8875 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC WB IP DB ICC/IF ICC/IF IHC-F IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | An extracellular fragment from the mouse p75 receptor (amino acids 43-161) |
| Description | Rabbit Polyclonal |
| Gene | NGFR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8875 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result).
Anti-p75 NGF Receptor antibody (ab8875) at 1/1000 dilution + HEK293 whole cell lysate at 30 µgSecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 75 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview
ab8875 staining p75 NGF Receptor in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 16 hour at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.See Abreview
Product References
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