Anti-PABP antibody
| Name | Anti-PABP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21060 |
| Prices | $404.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP FC WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human PABP |
| Description | Rabbit Polyclonal |
| Gene | PABPC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PABP antibody (ab21060) at 1 µg/ml + HeLa whole cell lysate at 20 µg/mlSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab21060 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab21060, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab21060 at 1/50 staining human HeLa cells by ICC/IF. HeLa cells fixed for 10 minutes with 1.9 % formaldehyde in 2 % sucrose in PBS then permeabilised for 10 minutes with 0.5% NP40 in PBS-10% sucrose. The cells were then blocked in horse serum and incubated with the antibody for 1 hour. An Alexa Fluor ® 555 conjugated donkey anti-rabbit antibody was used as the secondary.See Abreview
All lanes : Anti-PABP antibody (ab21060) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Brain (Mouse) Tissue Lysate Lane 4 : Pancreas (Mouse) Tissue Lysate (ab29363)Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue Lane 6 : Spinal Cord (Mouse) Tissue Lysate Lane 7 : Ovary (Mouse) Tissue Lysate - normal tissue Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 9 : Brain (Rat) Tissue Lysate - normal tissue Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlasab21060 staining PABP in Human appendix. The paraffin embedded tissue was incubated with ab21060 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab21060 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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