Anti-PABP2 antibody [EP3001Y]
| Name | Anti-PABP2 antibody [EP3001Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab81224 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP3001Y |
| Applications | WB IHC-P ICC/IF FC |
| Species Reactivities | Mouse, Bovine, Human |
| Antigen | Synthetic peptide from Human PABP2 |
| Description | Rabbit Monoclonal |
| Gene | PABPN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-PABP2 antibody [EP3001Y] (ab81224) at 1/20000 dilutionLane 1 : T47D cell lysatesLane 2 : SKBR3 cell lysatesLane 3 : Ramos cell lysatesLane 4 : Raji cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/6320_PABP2-Primary-antibodies-ab81224-3.jpg)
All lanes : Anti-PABP2 antibody [EP3001Y] (ab81224) at 1/20000 dilutionLane 1 : T47D cell lysatesLane 2 : SKBR3 cell lysatesLane 3 : Ramos cell lysatesLane 4 : Raji cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit at 1/2000 dilution
Immunohistochemistry staining of paraffin-embedded urinary bladder transitional cell carcinoma tissue, using 1/100 ab81224
Overlay histogram showing HeLa cells stained with ab81224 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81224, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
Amyotrophic lateral sclerosis-associated proteins TDP-43 and FUS/TLS function in - Amyotrophic lateral sclerosis-associated proteins TDP-43 and FUS/TLS function in
Kim SH, Shanware NP, Bowler MJ, Tibbetts RS. J Biol Chem. 2010 Oct 29;285(44):34097-105.
Amyotrophic lateral sclerosis-associated proteins TDP-43 and FUS/TLS function in - Amyotrophic lateral sclerosis-associated proteins TDP-43 and FUS/TLS function in
Kim SH, Shanware NP, Bowler MJ, Tibbetts RS. J Biol Chem. 2010 Oct 29;285(44):34097-105.