Anti-PABPN1 antibody [EP3000Y]

• Supplier: Abcam
• Catalog: ab75855
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP3000Y
• Applications: ICC/IF ICC/IF WB IP IHC-P FC
• Species Reactivities: Mouse, Human
• Antigen: A synthetic peptide corresponding to residues near the N-terminus of human PABPN1
• Description: Rabbit Monoclonal
• Gene: PABPN1
• Conjugate: Unconjugated

Product images

Lanes 1 - 2 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/200000 dilutionLane 3 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/1000000 dilutionLane 1 : Raw264.7 cell lysateLane 2 : MCF-7 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/1000 dilution

ab75855, at 1/100 dilution, staining PABPN1 in squamous cell cervical carcinoma, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

ab75855, at 1/100 dilution, staining PABPN1 in HeLa cells, by Immunofluorescent staining.

Overlay histogram showing MCF-7 cells stained with ab75855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75855, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II - 3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II

Davidson L, Muniz L, West S. Genes Dev. 2014 Feb 15;28(4):342-56.

Inhibition of U4 snRNA in human cells causes the stable retention of - Inhibition of U4 snRNA in human cells causes the stable retention of

Hett A, West S. PLoS One. 2014 May 5;9(5):e96174.

The human nuclear poly(a)-binding protein promotes RNA hyperadenylation and - The human nuclear poly(a)-binding protein promotes RNA hyperadenylation and

Bresson SM, Conrad NK. PLoS Genet. 2013;9(10):e1003893.

Tristetraprolin inhibits poly(A)-tail synthesis in nuclear mRNA that contains - Tristetraprolin inhibits poly(A)-tail synthesis in nuclear mRNA that contains

Su YL, Wang SC, Chiang PY, Lin NY, Shen YF, Chang GD, Chang CJ. PLoS One. 2012;7(7):e41313.

Poly(A) binding protein C1 is essential for efficient L1 retrotransposition and - Poly(A) binding protein C1 is essential for efficient L1 retrotransposition and

Dai L, Taylor MS, O'Donnell KA, Boeke JD. Mol Cell Biol. 2012 Nov;32(21):4323-36.

Depletion of nuclear poly(A) binding protein PABPN1 produces a compensatory - Depletion of nuclear poly(A) binding protein PABPN1 produces a compensatory

Bhattacharjee RB, Bag J. PLoS One. 2012;7(12):e53036.

Uncoordinated transcription and compromised muscle function in the lmna-null - Uncoordinated transcription and compromised muscle function in the lmna-null

Gnocchi VF, Scharner J, Huang Z, Brady K, Lee JS, White RB, Morgan JE, Sun YB, Ellis JA, Zammit PS. PLoS One. 2011 Feb 22;6(2):e16651.