ab151562 staining HeLa cells by ICC/IF. The cells were fixed with methanol. The cells were then stained with ab151562 at 1/200 in PBS for 1h at 22°C. An Alexa Fluro 488 goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. ab151562 produces a focal staining pattern that is enriched within the cytoplasm.See Abreview
All lanes : Anti-PACE4 antibody [EPR8320] (ab151562) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Flow cytometric analysis of permeabilized HeLa cells labeling PACE4 with ab151562 at 1/100 dilution (red), compared to a Rabbit IgG control antibody (green).