Anti-PAF1 antibody - ChIP Grade
| Name | Anti-PAF1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab20662 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ChIP |
| Species Reactivities | Mouse, Human, Rat, Dog, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PAF1 |
| Blocking Peptide | Human PAF1 peptide |
| Description | Rabbit Polyclonal |
| Gene | PAF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-PAF1 antibody - ChIP Grade (ab20662) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human PAF1 peptide (ab23389) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) (ab28446) at 1/10000 dilution
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 5µg of ab20662 (blue), and 20µl of Protein A/G sepharose beads in RIPA buffer. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH (active) and MYO-D and SERPINA (inactive) promoters and over the y-Actin gene (active). Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.
Product References
Histone H3R17me2a mark recruits human RNA polymerase-associated factor 1 complex - Histone H3R17me2a mark recruits human RNA polymerase-associated factor 1 complex
Wu J, Xu W. Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5675-80. doi:
Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR. - Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR.
Nakamura M, Basavarajaiah P, Rousset E, Beraud C, Latreille D, Henaoui IS, Lassot I, Mari B, Kiernan R. Retrovirology. 2012 Feb 8;9:13.
RNA polymerase II associated factor 1/PD2 maintains self-renewal by its - RNA polymerase II associated factor 1/PD2 maintains self-renewal by its
Ponnusamy MP, Deb S, Dey P, Chakraborty S, Rachagani S, Senapati S, Batra SK. Stem Cells. 2009 Dec;27(12):3001-11.