Anti-PAG antibody

• Supplier: Abcam
• Catalog: ab14989
• Prices: $379.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP IHC-P WB
• Species Reactivities: Mouse, Human
• Antigen: Recombinant fragment, corresponding to amino acids 97-432 of Human PAG
• Description: Rabbit Polyclonal
• Gene: PAG1
• Conjugate: Unconjugated

Product images

All lanes : Anti-PAG antibody (ab14989) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Lung (Human) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

IHC image of ab14989 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14989, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Differential transformation capacity of Src family kinases during the initiation - Differential transformation capacity of Src family kinases during the initiation

Cai H, Smith DA, Memarzadeh S, Lowell CA, Cooper JA, Witte ON. Proc Natl Acad Sci U S A. 2011 Apr 19;108(16):6579-84. doi:

Protein profiling of plasma membranes defines aberrant signaling pathways in - Protein profiling of plasma membranes defines aberrant signaling pathways in

Boyd RS, Jukes-Jones R, Walewska R, Brown D, Dyer MJ, Cain K. Mol Cell Proteomics. 2009 Jul;8(7):1501-15.