PAG1

Gene Symbol PAG1
Entrez Gene 55824
Alt Symbol CBP, PAG
Species Human
Gene Type protein-coding
Description phosphoprotein membrane anchor with glycosphingolipid microdomains 1
Other Description Csk-binding protein|phosphoprotein associated with glycosphingolipid microdomains 1|phosphoprotein associated with glycosphingolipid-enriched microdomains 1|transmembrane adapter protein PAG|transmembrane adaptor protein PAG|transmembrane phosphoprotein Cbp
Swissprots Q5BKU4 A8K1A3 Q9NYK0 Q9NWQ8 Q2M1Z9
Accessions EAW87085 EAW87086 Q9NWQ8 AA766638 AF240634 AAF67343 AK000680 BAA91321 AK095177 AK289818 BAF82507 BC090931 AAH90931 BC112159 AAI12160 BX426120 CB960651 EU832160 ACE87510 EU832254 ACE86824 XM_011517561 XP_011515863 XM_011517562 XP_011515864 XM_011517563 XP_011515865 XM_011517564 XP_011515866 XM_011517565 XP_011515867 XM_011517566 XP_011515868 XR_928792 NM_018440 NP_060910
Function Negatively regulates TCR (T-cell antigen receptor)- mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. {ECO:0000269|PubMed:10790433}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:10790433, ECO:0000269|PubMed:18070987}; Single-pass type III membrane protein {ECO:0000269|PubMed:10790433, ECO:0000269|PubMed:18070987}. Note=Present in lipid rafts.
Tissue Specificity Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level). {ECO:0000269|PubMed:10790433, ECO:0000269|PubMed:16160011}.

Cbp (H-100) - sc-25748 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cbp (H-100): sc-25748. Western blot analysis of Cbp expression in M1 whole cell lysate.

Cbp (G-8) - sc-365387 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cbp (G-8): sc-365387. Western blot analysis of Cbp expression in A-431 whole cell lysate.

Cbp (P-18) - sc-11667 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cbp (P-18): sc-11667. Western blot analysis of Cbp expression in HeLa (A) and A-431 (B) whole cell lysates.

Cbp (49) - sc-293026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cbp (49): sc-293026. Western blot analysis of Cbp expression in EB1 whole cell lysate.

Cbp (PAG-C1) - sc-51517 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Cbp (PAG-C1): sc-51517. Western blot analysis of Cbp expression in non-transfected: sc-117752 (A) and human Cbp transfected: sc-177032 (B) 293T whole cell lysates.

Anti-PAG antibody - ab14989 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAG antibody (ab14989) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Lung (Human) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-PAG antibody - ab128286 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PAG antibody (ab128286) at 1 µg/ml + 721_B cell lysate at 10 µgdeveloped using the ECL technique

Anti-PAG antibody [EPR9705] - ab155100 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9705
All lanes : Anti-PAG antibody [EPR9705] (ab155100) at 1/1000 dilutionLane 1 : Raji lysateLane 2 : Jurkat lysateLane 3 : HeLa lysateLane 4 : Human placenta lysateLysates/proteins at 10 µg per lane.

Anti-PAG antibody [MEM-255] - ab9527 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MEM-255
1. Frozen Raji cells lysate, immunoprecipitated with ab42062. Fresh Raji cells lysate, immunoprecipitated with ab42063. Frozen Raji cells lysate, immunoprecipitated with ab14989Reprobe with ab9527.The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.

Anti-PAG antibody [PAG-C1] - ab4206 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone PAG-C1
Overlay histogram showing Jurkat cells stained with ab4206 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4206, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PAG1 Antibody (C-Terminus) - LS-C14696 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PAG1 Antibody (aa97-432) - LS-C44850 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PAG1 Antibody (aa97-432) - LS-C14699 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAG1 Antibody (aa97-432) - LS-C63923 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAG1 Antibody (aa97-432) - LS-C78013 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PAG1 Antibody (clone MEM-255) - LS-C131193 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MEM-255

Anti-PAG1 Antibody (C-Terminus, clone PAG-C1) - LS-C131192 from LifeSpan Bioscience

Prices $560.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone PAG-C1

Anti-PAG1 Antibody (aa38-432, clone TS106) - LS-C191475 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone TS106

Anti-PAG1 Antibody (clone MEM-255) - LS-C202267 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MEM-255

Anti-PAG1 Antibody (aa97-432) - LS-C202773 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAG1 Antibody (C-Terminus, clone PAG-C1) - LS-C204149 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone PAG-C1

Anti-PAG1 Antibody (aa412-424) - LS-C345069 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAG1 Antibody (C-Terminus) - LS-C146554 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PAG1 / PAG antibody LS-C146554 Western blot of 721_B Cell lysate. Antibody concentration 1 ug/ml.

Anti-PAG1 Antibody (aa97-432) IHC-plus™ - LS-B2796 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PAG1 / PAG antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2796 concentration 10 ug/ml.

Anti-PAG1 Antibody (aa97-432, clone MEM-255) IHC-plus™ - LS-B2795 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MEM-255
Anti-PAG1 / PAG antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2795 concentration 10 ug/ml.
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