Anti-PAG antibody [PAG-C1]
| Name | Anti-PAG antibody [PAG-C1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab4206 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | PAG-C1 |
| Applications | IHC-P IP WB FC |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Synthetic C-teminal peptide (last 15 amino acids) (Human) conjugated to KLH |
| Description | Mouse Monoclonal |
| Gene | PAG1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing Jurkat cells stained with ab4206 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4206, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/6539_ab4206-3-ab4206FC.jpg)
Overlay histogram showing Jurkat cells stained with ab4206 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4206, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
1. Frozen Raji cells lysate, immunoprecipitated with ab4206 2. Fresh Raji cells lysate, immunoprecipitated with ab4206 3. Frozen Raji cells lysate, immunoprecipitated with ab14989Reprobe with ab9527.The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.
ab4206 staining PAG in human germinal center of lymphatic follicle and in dispersed T cells in appendix tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in citrate buffer pH 6.1. Th image show positive signal in T-cells.
Product References
Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src - Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src
Baumgartner M, Angelisova P, Setterblad N, Mooney N, Werling D, Horejsi V, Langsley G. Blood. 2003 Mar 1;101(5):1874-81. Epub 2002 Oct 31.
Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a - Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a
Vang T, Abrahamsen H, Myklebust S, Horejsi V, Tasken K. J Biol Chem. 2003 May 16;278(20):17597-600. Epub 2003 Mar 28.