All lanes : Anti-PAK2 (phospho S20) antibody (ab59359) at 1/500 dilutionLane 1 : Extracts from 293 cells, treated with Sorbitol (0.4M, 30min) with no immunizing peptideLane 2 : Extracts from 293 cells, treated with Sorbitol (0.4M, 30mins) with immunizing peptideObserved band size : 58 kDa (why is the actual band size different from the predicted?)
ab59359, at a 1/50 dilution, staining human PAK2 (phospho S20) in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).