PAK2

Gene Symbol PAK2
Entrez Gene 5062
Alt Symbol PAK65, PAKgamma
Species Human
Gene Type protein-coding
Description p21 protein (Cdc42/Rac)-activated kinase 2
Other Description PAK-2|S6/H4 kinase|gamma-PAK|p21 (CDKN1A)-activated kinase 2|p21-activated kinase 2|p58|serine/threonine-protein kinase PAK 2
Swissprots Q6ISC3 Q13177 Q13154
Accessions AFG72980 Q13177 AI140979 AK291339 BAF84028 BC031667 BC046197 BC063539 BC069613 AAH69613 BG572555 CA445078 DB164364 DQ786200 U24153 AAA65442 U25975 AAA75468 XM_011512870 XP_011511172 NM_002577 NP_002568
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the o
Subcellular Location Serine/threonine-protein kinase PAK 2: Cytoplasm. Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.
Tissue Specificity Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Top Pathways Axon guidance, Focal adhesion, T cell receptor signaling pathway, Renal cell carcinoma, MAPK signaling pathway

Phospho-PAK2 (Ser20) Antibody - 2607 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP for the indicated times, using Phospho-PAK2 (Ser20) Antibody (upper) or PAK1/2/3 Antibody #2604 (lower).

PAK2 Antibody - 2608 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3, C6, COS, guinea pig neutrophils (GPN) and HEK293 cells, using PAK2 Antibody.

PAK2 (C17A10) Rabbit mAb - 2615 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C17A10
Western blot analysis of extracts from 293, C2C12 and COS cells using PAK2 (C17A10) Rabbit mAb.

PAK2 (3B5) Mouse mAb - 4825 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3B5
Western blot analysis of extracts from various cell types using PAK2 (3B5) Mouse mAb.

p-α/β/γPAK (Thr 402) - sc-12940 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-α/β/γPAK (Thr 402)-R: sc-12940-R. Western blot analysis of α/β/γPAK phosphorylation in untreated (A), forskolin treated (B) and forskolin and lambda protein phosphatase treated (C) SK-N-MC whole cell lysates.

γPAK (E-9) - sc-373740 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γPAK (E-9): sc-373740. Western blot analysis of γPAK expression in JAR whole cell lysate.

γPAK (F-12) - sc-133113 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γPAK (F-12): sc-133113. Western blot analysis of γPAK expression in Jurkat whole cell lysate.

γPAK (H-4) - sc-377194 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γPAK (H-4): sc-377194. Western blot analysis of γPAK expression in Jurkat whole cell lysate.

γPAK (N-19) - sc-1872 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
γPAK (N-19): sc-1872. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in germinal centers and cells in non-germinal centers.

γPAK (C-19) - sc-1519 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
γPAK (C-19): sc-1519. Western blot analysis of γPAK expression in Jurkat whole cell lysate (A) and rat brain tissue extract (B).

γPAK (G-10) - sc-137208 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
γPAK (G-10): sc-137208. Western blot analysis of γPAK expression in Jurkat (A) and SK-N-MC (B) whole cell lysates and rat brain tissue extract (C).

γPAK (V-19) - sc-7117 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
γPAK siRNA (h): sc-36183. Western blot analysis of γPAK expression in non-transfected control (A) and γPAK siRNA transfected (B) HeLa cells. Blot probed with γPAK (V-19): sc-7117. GAPDH (FL-335): sc-25778 used as specificity and loading control.

p-γPAK (Ser 141) - sc-16775 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-PAK (Ser 141): sc-16775. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic and nuclear staining of cells in germinal centers and cells in non-germinal centers.

Anti-PAK2 (phospho S192) antibody - ab79505 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAK2 (phospho S192) antibody (ab79505) at 1/500 dilutionLane 1 : Extracts from HeLa cellsLane 2 : Extracts from HeLa cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-PAK2 (phospho S197) antibody - ab111850 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111850 at 1/50 dilution staining PAK2 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-PAK2 (phospho S20) antibody - ab59359 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAK2 (phospho S20) antibody (ab59359) at 1/500 dilutionLane 1 : Extracts from 293 cells, treated with Sorbitol (0.4M, 30min) with no immunizing peptideLane 2 : Extracts from 293 cells, treated with Sorbitol (0.4M, 30mins) with immunizing peptideObserved band size : 58 kDa (why is the actual band size different from the predicted?)

Anti-PAK2 (phospho S20) antibody [EPR658(2)] - ab76419 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR658(2)
All lanes : Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate treated with calyculin ALysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution

Anti-PAK2 antibody - ab45426 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-PAK2 antibody (ab45426) at 0.5 µg/mlLane 2 : Anti-PAK2 antibody (ab45426) at 1 µg/mlLane 1 : Jurkat lysateLane 2 : Jurkat lysateLysates/proteins at 15 µg per lane.Secondaryanti-rabbit HRP

Anti-PAK2 antibody - ab168989 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-PAK2 antibody (ab168989) at 1/500 dilutionLane 1 : PAK2 transfected 293T cell lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-PAK2 antibody - ab38849 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PAK2 antibody (ab38849) + Ovary cell lysate

Anti-PAK2 antibody - ab19010 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAK2 antibody - ab196952 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAK2 antibody (ab196952) at 1/500 dilutionLane 1 : HeLa cells, treated with TSA (400nM, 24hours)Lane 2 : HeLa cells, treated with TSA (400nM, 24 hours) and synthesized peptide

Anti-PAK2 antibody - ab196958 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAK2 antibody (ab196958) at 1/500 dilutionLane 1 : HT29 cell extractLane 2 : HT29 cell extract with blocking peptide

Anti-PAK2 antibody - ab196961 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAK2 antibody (ab196961) at 1/500 dilutionLane 1 : HepG2 cell extracts, treated with serum (20%, 15mins)Lane 2 : HepG2 cell extracts, treated with serum (20%, 15mins) with synthesized peptide

Anti-PAK2 antibody - ab1179 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Western blot of 10 µg HEK 293 cell extracts with Anti-AK (GLO151). The arrow denotes the position corresponding to AK (~ 36 kDa).
Search more