Anti-PAK4 antibody
| Name | Anti-PAK4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab62509 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human |
| Antigen | A 13 amino acid synthetic peptide from near the center of human PAK4 |
| Description | Rabbit Polyclonal |
| Gene | PAK4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/mlLane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/mlLane 1 : SW480 cell lysateLane 2 : SW480 cell lysateLysates/proteins at 15 µg per lane.
Immunohistochemistry of PAK4 in paraffin embedded human colon tissue section with ab62509 antibody at 10 µg/ml.
ICC/IF image of ab62509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of PAK4 in Human Colon cells using ab62509 at 20 ug/ml.
Product References
ROCK1 and LIM kinase modulate retrovirus particle release and cell-cell - ROCK1 and LIM kinase modulate retrovirus particle release and cell-cell
Wen X, Ding L, Wang JJ, Qi M, Hammonds J, Chu H, Chen X, Hunter E, Spearman P. J Virol. 2014 Jun;88(12):6906-21.
MiR-145 regulates PAK4 via the MAPK pathway and exhibits an antitumor effect in - MiR-145 regulates PAK4 via the MAPK pathway and exhibits an antitumor effect in
Wang Z, Zhang X, Yang Z, Du H, Wu Z, Gong J, Yan J, Zheng Q. Biochem Biophys Res Commun. 2012 Oct 26;427(3):444-9. doi:
P21-activated kinase 4 overexpression in metastatic gastric cancer patients. - P21-activated kinase 4 overexpression in metastatic gastric cancer patients.
Ahn HK, Jang J, Lee J, Se Hoon P, Park JO, Park YS, Lim HY, Kim KM, Kang WK. Transl Oncol. 2011 Dec;4(6):345-9. Epub 2011 Dec 1.