PAK4

Gene Symbol PAK4
Entrez Gene 10298
Alt Symbol -
Species Human
Gene Type protein-coding
Description p21 protein (Cdc42/Rac)-activated kinase 4
Other Description PAK-4|p21(CDKN1A)-activated kinase 4|p21-activated kinase 4|protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs|serine/threonine-protein kinase PAK 4
Swissprots Q8N4E1 O96013 Q9BU33 Q9ULS8 Q8NCH5 B4DGG6 Q8NDE3
Accessions EAW56858 EAW56859 EAW56860 EAW56861 EAW56862 EAW56863 EAW56864 EAW56865 EAW56866 O96013 AB032968 BAA86456 AF005046 AAD01210 AJ011855 CAA09820 AK074728 BAC11166 AK291839 BAF84528 AK293517 BAG56999 AK294586 BAG57777 AK300632 BAG62322 AK304651 BAG65427 AL834236 CAD38914 AM392622 CAL37500 AM392751 CAL37629 AM393512 CAL38389 AX775863 CAE11631 AX775865 CAE11632 AX775867 CAE11633 AX775869 CAE11634 AX775871 CAE11635 BC002921 AAH02921 BC011368 AAH11368 BC025282 AAH25282 BC034511 AAH34511 BG773480 BM129363 DQ891039 ABM81965 DQ894218 ABM85144 GQ129406 ACT64540 GQ129407 ACT64541 XM_011526316 XP_011524618 XM_011526317 XP_011524619 XM_011526318 XP_011524620 XM_011526319 XP_011524621 XM_011526320 XP_011524622 NM_001014831 NP_001014831 NM_001014832 NP_001014832 NM_001014834 NP_001014834 NM_001014835 NP_001014835 NM_005884 NP_005875 NM_001014833
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell
Subcellular Location Cytoplasm {ECO:0000269|PubMed:12356872}. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways.
Tissue Specificity Highest expression in prostate, testis and colon.
Top Pathways Axon guidance, Focal adhesion, T cell receptor signaling pathway, Renal cell carcinoma, ErbB signaling pathway

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Antibody - 3241 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP for the indicated times, using Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Antibody. (Provided by Drs. Qian Zhan and John Badwey, Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts.)

PAK4 Antibody - 3242 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SH-SY5Y, NIH/3T3, C6 and COS cells and guinea pig neutrophils, using PAK4 Antibody.

PAK4 (H-140) - sc-28779 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PAK4 (H-140): sc-28779. Western blot analysis of PAK4 expression in non-transfected: sc-110760 (A) and human PAK4 transfected: sc-111101 (B) 293 whole cell lysates.

PAK4 (P-21) - sc-18622 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PAK4 (P-21): sc-18622. Western blot analysis of PAK4 expression in non-transfected: sc-110760 (A) and human PAK4 transfected: sc-111101 (B) 293 whole cell lysates.

PAK4 (6C1) - sc-81532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PAK4 (6C1): sc-81532. Western blot analysis of PAK4 expression in non-transfected: sc-110760 (A) and human PAK4 transfected: sc-111101 (B) 293 whole cell lysates.

PAK4 (B-3) - sc-390507 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PAK4 (B-3): sc-390507. Western blot analysis of PAK4 expression in non-transfected: sc-117752 (A) and human PAK4 transfected: sc-173381 (B) 293T whole cell lysates.

PAK4 (B-6) - sc-393367 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PAK4 (B-6): sc-393367. Western blot analysis of PAK4 expression in NTERA-2 cl.D1 (A), SH-SY5Y (B) and NIH/3T3 (C) whole cell lysates.

p-PAK4 (71.Ser 474) - sc-135774 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PAK4 phosphorylation in non-transfected: sc-110760 (A,D), untreated human PAK4 transfected: sc-111101 (B,E) and lambda protein phosphatase (sc-200312A) treated human PAK4 transfected: sc-111101 (C,F) 293 whole cell lysates. Antibodies tested include p-PAK4 (71.Ser 474): sc-135774 (A,B,C) and PAK4 (6C1): sc-81532 (D,E,F).

p-PAK4 (93.Ser 474) - sc-135775 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PAK4 phosphorylation in non-transfected: sc-110760 (A,D), untreated human PAK4 transfected: sc-111101 (B,E) and lambda protein phosphatase (sc-200312A) treated human PAK4 transfected: sc-111101 (C,F) 293 whole cell lysates. Antibodies tested include p-PAK4 (93.Ser 474): sc-135775 (A,B,C) and PAK4 (6C1): sc-81532 (D,E,F).

Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] - ab134097 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2471(2)
Overlay histogram showing Hela cells stained with ab134097 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134097, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PAK4 antibody - ab62509 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/mlLane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/mlLane 1 : SW480 cell lysateLane 2 : SW480 cell lysateLysates/proteins at 15 µg per lane.

Anti-PAK4 antibody - ab19007 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PAK4 antibody (ab19007) at 1 µg/ml + COS cell lysate

Anti-PAK4 antibody - ab173315 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PAK4 antibody (ab173315) at 1/100 dilution + Neuro-2a cell line lysates at 35 µg

Anti-PAK4 antibody - ab137960 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PAK4 antibody (ab137960) at 1/500 dilution + Jurkat cell lysate at 30 µg

Anti-PAK4 antibody - ab56591 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56591 PAK4 antibody at 1ug/ml.

Anti-PAK4 Antibody (N-Terminus) - LS-C9450 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK4 Antibody (N-Terminus) - LS-C38048 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-PAK4 Antibody (aa1-120) - LS-C38050 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-PAK4 Antibody (phospho-Ser474) - LS-C47727 from LifeSpan Bioscience

Prices $285.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Anti-PAK4 Antibody (aa470-485) - LS-C189149 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PAK4 Antibody (aa186-206, APC) - LS-C223585 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK4 Antibody (aa186-206, Biotin) - LS-C223586 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK4 Antibody (aa186-206, AP) - LS-C223584 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK4 Antibody (aa186-206, FITC) - LS-C223587 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK4 Antibody (aa186-206, Azide-free, HRP) - LS-C223588 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more