Lanes 1 - 4 : Anti-PAK6 antibody (ab41431) at 1/1000 dilutionLanes 5 - 8 : Anti-PAK6 (phospho S165) antibody (ab41432) at 1/1000 dilutionLane 1 : Human PAK6 recombinant protein phosphorylated by ERK2Lane 2 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing Lane 3 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific peptide Lane 4 : Human PAK6 recombinant protein phosphorylated by ERK2 with PAK6 N-terminal peptide (ab41431 immunogen) Lane 5 : Human PAK6 recombinant protein phosphorylated by ERK2Lane 6 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probingLane 7 : Human PAK6 recombinant protein phosphorylated by ERK2 with phospho-S165-PAK6 peptide (ab41432 immunogen) Lane 8 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific phospho-serine peptide