Anti-pan Cadherin antibody [CH-19]

• Supplier: Abcam
• Catalog: ab6528
• Prices: $400.00
• Sizes: 500 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: CH-19
• Applications: FC ICC/IF ICC/IF IHC-P WB IHC-F ICC/IF
• Species Reactivities: Mouse, Rat, Rabbit, Goat, Chicken, Guinea Pig, Hamster, Bovine, Cat, Dog, Human, Pig, Xenopus, Snake, Zebrafish
• Antigen: Synthetic peptide: DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH, corresponding to amino acids 889-912 of Chicken N-Cadherin (cdh2)
• Description: Mouse Monoclonal
• Gene: CDH3
• Conjugate: Unconjugated

Product images

Immunofluorescent imaging of human cells (U2OS) with ab6528 reveals the expected highly specific membranous distribution. IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C.

Ab6528 positively staining formaldehyde fixed HEK 293 cells at 1/200 in conjunction with goat anti mouse (Alexa 546) 1/1500. This image is an edited version of an image received courtesy of an Abreview submitted by Kun Liu on 19 September 2005. We do not have any further information relating to this image.See Abreview

ab6528 (1/1500) positively staining cadehrin in mouse kidney tissue sections, as secondary antibody goat anti mouse biotin conjugated was used. Please see accompanying abreview for additional information.See Abreview

ab6528 staining pan Cadherin in mouse Cor1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with TBS, BSA, azide and Triton and blocking with 1% BSA  for 30 minutes at RT was performed. Samples were incubated with primary antibody (1/1500: in TBS, BSA, azide and 0.3%Triton) for 2 hours. An Alexa Fluor®546-conjugated goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution. The image was captured using Z-stacks/Zeiss Apotome microscope rig.See Abreview

All lanes : Anti-pan Cadherin antibody [CH-19] (ab6528) at 1/2000 dilutionLane 1 : ARPE-19 cells, membrane fractionLane 2 : HEK 293 cells, membrane fractionLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse conjugated to HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-pan Cadherin antibody [CH-19] (ab6528) at 1/2000 dilutionLane 1 : Porcine kidney extract - whole cell lysateLane 2 : Porcine RPE cells - whole cell lysateLysates/proteins at 25 µg per lane.SecondaryAn HRP-conjugated goat polyclonal to mouse IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Overlay histogram showing HeLa cells stained with ab6528 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6528, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

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