Anti-pan Cadherin antibody - Plasma Membrane Marker

• Supplier: Abcam
• Catalog: ab16505
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF ICC/IF WB IHC-P IHC-F
• Species Reactivities: Mouse, Rat, Human, Zebrafish, Chicken, Bovine, Xenopus
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human pan Cadherin
• Blocking Peptide: Human pan Cadherin peptide
• Description: Rabbit Polyclonal
• Gene: CDH3
• Conjugate: Unconjugated

Product images

All lanes : Anti-pan Cadherin antibody - Plasma Membrane Marker (ab16505) at 1 µg/mlLane 1 : Mouse heartLane 2 : HeLa cell lysateLane 3 : 3T3 cell lysateLane 4 : Mouse muscleLane 5 : Human heartLane 6 : Mouse heart with Human pan Cadherin peptide (ab17098) at 1 µg/mlLane 7 : HeLa cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/mlLane 8 : 3T3 cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/mlLane 9 : Mouse muscle with Human pan Cadherin peptide (ab17098) at 1 µg/mlLane 10 : Human heart with Human pan Cadherin peptide (ab17098) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit conjugated to Alexafluor 680 at 1/10000 dilutionPerformed under reducing conditions.

HeLa cells fixed in methanol and stained with ab16505 (2µg/ml). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS. The 2 images show the cells stained with different secondary antibodies, Donkey anti Rabbit FITC (image A) and Donkey anti Rabbit Cy3 (image B). In each case ab16505 stains the plasma membrane.  In image A ab16505 is stained green and in image B ab16505 is stained red. In both images the DNA is stained with DAPI (blue).

ICC/IF image of ab16505 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16505, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

IHC image of pan Cadherin staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16505, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescence analysis of HeLa cells, staining pan Cadherin (red) with ab16505. Cells were fixed with paraformaldehyde, permeabilized in methanol and blocked for 1 hour at room temperature in DPBS containing 2 mg/mL BSA, 1% fish gelatin, 0.1% Triton-X 100 and 5% goat serum. Cells were then incubated for 1 hour at room temperature with the primary antibody diluted in blocking buffer. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

All lanes : Anti-pan Cadherin antibody - Plasma Membrane Marker (ab16505) at 1 µg/mlLane 1 : Human heart lysateLane 2 : Mouse heart lysateLane 3 : Rat heart lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

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