Anti-pan PDK antibody [3H5BE12]

• Supplier: Abcam
• Catalog: ab115321
• Prices: $359.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 3H5BE12
• Applications: IHC-P WB
• Species Reactivities: Human
• Antigen: Recombinant full length protein
• Description: Mouse Monoclonal
• Gene: PDK1
• Conjugate: Unconjugated

Product images

All lanes : Anti-pan PDK antibody [3H5BE12] (ab115321) at 1 µg/mlLane 1 : human heart mitochondria at 20 µgLane 2 : human liver mitochondria at 10 µgLane 3 : rat liver mitochondria at 10 µgLane 4 : mouse liver mitochondria at 10 µgLane 5 : PDK1 recombinant protein ab110359 at 0.1 µgLane 6 : PDK2 recombinant protein ab110354 at 0.1 µgLane 7 : PDK3 recombinant protein ab110355 at 0.1 µgLane 8 : PDK4 recombinant protein ab110356 at 0.1 µgLane 9 : PDK1 recombinant protein ab86986 at 0.1 µgSecondaryGAM IR 800 1:5000Performed under reducing conditions.

IHC image of ab115321 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

developed using the ECL techniquePerformed under reducing conditions.