Anti-Pannexin 1 antibody
| Name | Anti-Pannexin 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab124131 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mouse, Rabbit, Pig, Monkey, Ape, Hamster, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Pannexin 1 |
| Description | Rabbit Polyclonal |
| Gene | PANX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Pannexin 1 antibody (ab124131) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab124131 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab124131, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa and HepG2 cells at 1µg/ml
Product References
Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP - Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP
Pinheiro AR, Paramos-de-Carvalho D, Certal M, Costa C, Magalhaes-Cardoso MT, Ferreirinha F, Costa MA, Correia-de-Sa P. Cell Commun Signal. 2013 Sep 18;11:70.
ATP released via pannexin-1 hemichannels mediates bladder overactivity triggered - ATP released via pannexin-1 hemichannels mediates bladder overactivity triggered
Timoteo MA, Carneiro I, Silva I, Noronha-Matos JB, Ferreirinha F, Silva-Ramos M, Correia-de-Sa P. Biochem Pharmacol. 2014 Jan 15;87(2):371-9.