All lanes : Anti-PAPP A2 antibody (ab59097) at 1/1000 dilutionLane 1 : Whole cell lyste prepared from human breast cancer MDA-MB-231 cells (treated with SF)Lane 2 : Whole cell lyste prepared from human breast cancer MDA-MB-231 cells (treated with TPA)Lane 3 : Whole cell lyste prepared from human breast cancer MDA-MB-231 cells (treated with IL1-beta)Lane 4 : Whole cell lyste prepared from human breast cancer MDA-MB-231 cells (treated with TNF-alpha)Lane 5 : Whole cell lyste prepared from human breast cancer MDA-MB-231 cells (treated with Testosterone)Lysates/proteins at 20 µl per lane.