Anti-PAPSS2 antibody
| Name | Anti-PAPSS2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56393 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: DPAGMPHPET KKDLYEPTHG GKVLSMAPGL TSVEIIPFRV AAYNKAKKAM DFYDPARHNE FDFISGTRMR KLAREGENPP DGFMAPKAWK VLTDYYRSLE , corresponding to amino acids 513-613 of Human PAPSS2 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | PAPSS2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab56393 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56393, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7151_ab56393-3-ab56393FC.jpg)
Overlay histogram showing HeLa cells stained with ab56393 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56393, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
PAPSS2 antibody (ab56393) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
PAPSS2 antibody (ab56393) at 1ug/lane + A-431 cell lysate at 25ug/lane.