PAPSS2

Gene Symbol PAPSS2
Entrez Gene 9060
Alt Symbol ATPSK2, BCYM4, SK2
Species Human
Gene Type protein-coding
Description 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Other Description 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 2|ATP sulfurylase/APS kinase 2|ATP sulfurylase/adenosine 5'-phosphosulfate kinase|PAPS synthase 2|PAPS synthetase 2|SK 2|bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2|bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2
Swissprots Q9UP30 Q9UHM1 Q9P0G6 O95340 Q9BZL2 Q9UKD3
Accessions AAF40307 EAW50181 EAW50182 EAW50183 O95340 AF074331 AAD38423 AF091242 AAC64583 AF150754 AAF20366 AF173365 AAF12761 AF313907 AAK00296 AK301453 BAG62977 AK309262 AK312556 BAG35453 BC009894 AAH09894 BQ221395 BQ772647 BQ888954 CB960618 DQ893625 ABM84551 DQ895430 ABM86356 NM_001015880 NP_001015880 NM_004670 NP_004661
Function Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. May have a important role in skeletogenesis during postnatal growth (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in cartilage and adrenal gland. {ECO:0000269|PubMed:19474428}.
Top Pathways Sulfur metabolism, Purine metabolism, Selenocompound metabolism

PAPSS 1/2 (A-10) - sc-271429 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PAPSS 1/2 (A-10): sc-271429. Western blot analysis of PAPSS 1/2 expression in Ramos (A) and MDA-MB-231 (B) whole cell lysates.

PAPSS 2 (C-15) - sc-47309 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PAPSS 2 (C-15): sc-47309. Western blot analysis of PAPSS 2 expression in MDA-MB-231 (A) and NIH/3T3 (B) whole cell lysates.

PAPSS 2 (H-34) - sc-135162 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

PAPSS 2 (C-13) - sc-47308 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PAPSS 2 (C-13): sc-47308. Western blot analysis of PAPSS 2 expression in PC-12 (A), MDA-MB-231 (B) and NIH/3T3 (C) whole cell lysates.

PAPSS 2 (N-16) - sc-47311 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PAPSS 2 (SQ-19) - sc-100801 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
PAPSS 2 (SQ-19): sc-100801. Western blot analysis of PAPSS 2 expression in A-431 whole cell lysate.

Anti-PAPSS1 antibody - ab56398 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab56398 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56398, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-PAPSS1 antibody - C-terminal - ab135769 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PAPSS1 antibody - C-terminal (ab135769) at 1/100 dilution + A2058 cell line lysates at 35 µgdeveloped using the ECL technique

Anti-PAPSS2 antibody - ab88609 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PAPSS2 antibody (ab88609) at 1 µg/ml + human liver lysate at 50 µg

Anti-PAPSS2 antibody - ab56393 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab56393 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56393, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-PAPSS2 antibody - ab37611 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PAPSS2 antibody (ab37611) at 1/100 dilution + Jurkat cell lysate

Anti-PAPSS2 Antibody (C-Terminus, APC) - LS-C220257 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (C-Terminus, AP) - LS-C220256 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (C-Terminus, Biotin) - LS-C220258 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (C-Terminus, FITC) - LS-C220259 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (N-Terminus, AP) - LS-C220262 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (N-Terminus, APC) - LS-C220263 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (C-Terminus, Azide-free, HRP) - LS-C220260 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (C-Terminus, PE) - LS-C220261 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (N-Terminus, Biotin) - LS-C220264 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (C-Terminus) - LS-C81273 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PAPSS2 Antibody (N-Terminus, FITC) - LS-C220265 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (N-Terminus, PE) - LS-C220267 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (N-Terminus, Azide-free, HRP) - LS-C220266 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPSS2 Antibody (C-Terminus) - LS-C328146 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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