Anti-PAR4 antibody
| Name | Anti-PAR4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5787 |
| Prices | $380.00 |
| Sizes | 200 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human PAR4 aa 324-342 |
| Description | Rabbit Polyclonal |
| Gene | PAWR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab5787 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5787 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of paraffin-embedded mouse embryonic corneal tissue, staining PAR4 with ab5787.Embryos were fixed with 4% paraformaldehyde and treated for antigen retrieval by boiling for 10 min in the citrate buffer (pH 6.0). Sections were incubated with primary antibody and a biotinylated anti-rabbit secondary antibody.
Product References
A complex regulatory network of transcription factors critical for ocular - A complex regulatory network of transcription factors critical for ocular
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Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic - Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic
Fernandez-Marcos PJ, Abu-Baker S, Joshi J, Galvez A, Castilla EA, Canamero M, Collado M, Saez C, Moreno-Bueno G, Palacios J, Leitges M, Serrano M, Moscat J, Diaz-Meco MT. Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12962-7. doi:
Human PRKC apoptosis WT1 regulator is a novel PITX2-interacting protein that - Human PRKC apoptosis WT1 regulator is a novel PITX2-interacting protein that
Acharya M, Lingenfelter DJ, Huang L, Gage PJ, Walter MA. J Biol Chem. 2009 Dec 11;284(50):34829-38.
IGF-I inhibition of apoptosis is associated with decreased expression of prostate - IGF-I inhibition of apoptosis is associated with decreased expression of prostate
Chung H, Seo S, Moon M, Park S. J Endocrinol. 2007 Jul;194(1):77-85.