PAWR

Gene Symbol PAWR
Entrez Gene 5074
Alt Symbol PAR4, Par-4
Species Human
Gene Type protein-coding
Description PRKC, apoptosis, WT1, regulator
Other Description PRKC apoptosis WT1 regulator protein|WT1-interacting protein|prostate apoptosis response 4 protein|prostate apoptosis response protein 4|prostate apoptosis response protein PAR-4|prostate apoptosis response-4|transcriptional repressor PAR4
Swissprots O75796 Q6FHY9 Q96IZ0 Q8N700
Accessions AAM27453 AAN23144 AAP43693 CCQ43185 EAW97346 EAW97347 EAW97348 EAW97349 Q96IZ0 AK027848 AK095165 BC007018 AAH07018 BC009637 CA313080 CN280333 CR536549 CAG38786 U63809 AAC24947 XM_006719435 XP_006719498 XM_006719436 XP_006719499 XR_944560 XR_944561 NM_002583 NP_002574
Function Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down- regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. {ECO:0000269|PubMed:11585763}.
Subcellular Location Cytoplasm. Nucleus. Note=Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies. {ECO:0000250}.
Tissue Specificity Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers. {ECO:0000269|PubMed:8943350}.

Phospho-PAR-4 (Thr163) Antibody - 2329 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or forskolin-treated (30 μM), using Phospho-PAR-4 (Thr163) Antibody (upper) or total PAR-4 Antibody #2328 (lower).

PAR-4 Antibody - 2328 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SW620, ACHN, LNCaP and A20 cell lines, using PAR-4 Antibody.

PAR4 (R-334) - sc-1807 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PAR4 (R-334): sc-1807. Western blot analysis of PAR4 expression in AT-3 whole cell lysate (A) and 20 ng PAR4 polyhistidine fusion protein (B).

PAR4 (C-19) - sc-1249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PAR4 (C-19): sc-1249. Western blot analysis of PAR4 expression in AT-3 whole cell lysate.

PAR4 (A-10) - sc-1666 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PAR4 (A-10): sc-1666. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue. Note nuclear staining of glandular epithelial cells.

PAR4 (3G9H7) - sc-130078 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PAR4 (3G9H7): sc-130078. Western blot analysis of PAR4 expression in non-transfected: sc-110760 (A) and mouse PAR4 transfected: sc-179292 (B) 293 whole cell lysates.

PAR4 (4H12E9) - sc-130079 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PAR4 (4H12E9): sc-130079. Western blot analysis of full-length human recombinant PAR4-Trx protein (A) and PAR4 expression in HeLa whole cell lysate (B).

Anti-PAR4 antibody - ab5787 from Abcam

Prices $380.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab5787 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5787 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PAR4 antibody - ab49155 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PAR4 antibody (ab49155) at 2 µg/ml + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000

Anti-PAR4 antibody - ab128253 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAR4 antibody - ab53718 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAR4 antibody (ab53718) at 1/500 dilutionLane 1 : Extracts from NIH/3T3 cells, minus immunising peptideLane 2 : Extracts from NIH/3T3 cells, plus immunising peptide

Anti-PAR4 antibody - ab75686 from Abcam

Prices $370.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab75686, at 1/100 dilution, staining PAR4 in Human heart by immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Anti-PAR4 antibody - ab3949 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAR4 antibody [3G9H7,4H12E9] - ab70400 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3G9H7,4H12E9
All lanes : Anti-PAR4 antibody [3G9H7,4H12E9] (ab70400) at 1/500 dilutionLane 1 : Molecular weight markersLane 2 : Full length Trx-PAR4 recombinant proteinLane 3 : HeLa cell lysate

Anti-PAR4 antibody [EPR3991] - ab92590 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3991
All lanes : Anti-PAR4 antibody [EPR3991] (ab92590) at 1/10000 dilutionLane 1 : LNCap cell lysateLane 2 : Jurkat cell lysateLane 3 : A549 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PAR4 antibody [EPR3992] - ab109174 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3992
All lanes : Anti-PAR4 antibody [EPR3992] (ab109174) at 1/1000 dilutionLane 1 : LnCaP cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : A549 cell lysateLysates/proteins at 10 µg per lane.

Anti-PAR4 antibody, prediluted - ab15511 from Abcam

Prices $370.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Ab15511 staining PAR4 in human heart by Immunohistochemistry (formalin/paraffin).

Anti-PAWR / PAR4 Antibody - LS-C178390 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAWR / PAR4 Antibody (aa291-340) IHC-plus™ - LS-B7615 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PAWR / PAR4 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7615 concentration 5 ug/ml.

Anti-PAWR / PAR4 Antibody (clone 3G9H7,4H12E9) IHC-plus™ - LS-B4094 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3G9H7,4H12E9
Anti-PAWR / PAR4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4094 dilution 1:200.

Anti-PAWR / PAR4 Antibody - LS-C25972 from LifeSpan Bioscience

Prices $325.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PAWR / PAR4 Antibody - LS-C40650 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAWR / PAR4 Antibody - LS-C89370 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAWR / PAR4 Antibody (Internal) - LS-C153404 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAWR Antibody (Internal, clone EPR3991) - LS-C105758 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3991
Western blot analysis on (A) LNCap, (B) Jurkat, (C) A549, and (D) HeLa cell lysates using anti-PAR4 antibody (LS-C105758).
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