Anti-PAR6 antibody

• Supplier: Abcam
• Catalog: ab49776
• Prices: $378.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human PAR6
• Description: Rabbit Polyclonal
• Gene: PARD6A
• Conjugate: Unconjugated

Product images

Anti-PAR6 antibody (ab49776) at 1 µg/ml + PARD6A partial Human Recombinant Tagged Protein at 0.2 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab49776 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49776, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HEK 293 cells.

Product References

CD24 regulated gene expression and distribution of tight junction proteins is - CD24 regulated gene expression and distribution of tight junction proteins is

Ye P, Nadkarni MA, Simonian M, Hunter N. BMC Cell Biol. 2009 Jan 13;10:2.

Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional - Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional

Koch S, Capaldo CT, Samarin S, Nava P, Neumaier I, Skerra A, Sacks DB, Parkos CA, Nusrat A. Mol Biol Cell. 2009 Nov;20(22):4816-25.