![Overlay histogram showing HepG2 cells stained with ab104832 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104832, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7232_ab104832-4-ab104832FC.jpg)
Overlay histogram showing HepG2 cells stained with ab104832 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104832, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-Parathyroid Hormone Receptor 1 antibody [4D2] (ab104832) at 1/500 dilution + Parathyroid Hormone Receptor 1-hIgGFc transfected HEK293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7233_Parathyroid-Hormone-Receptor-1-Primary-antibodies-ab104832-1.jpg)
Anti-Parathyroid Hormone Receptor 1 antibody [4D2] (ab104832) at 1/500 dilution + Parathyroid Hormone Receptor 1-hIgGFc transfected HEK293 cell lysate
ab104832 at 1/200 dilution staining Parathyroid Hormone Receptor 1 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
ab104832 staining Parathyroid Hormone Receptor 1 in SK-BR-3 cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.