Anti-PARK7/DJ1 antibody [EP2816Y]
| Name | Anti-PARK7/DJ1 antibody [EP2816Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab76241 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP2816Y |
| Applications | WB IHC-P ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide derived from an internal region of human DJ1 |
| Description | Rabbit Monoclonal |
| Gene | PARK7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing Jurkat cells stained with ab76241 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76241, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241) at 1/20000 dilutionLane 1 : TF-1 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7311_dj1.gif)
All lanes : Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241) at 1/20000 dilutionLane 1 : TF-1 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab76241 at 1/100 dilution.
Product References
Use of cysteine-reactive cross-linkers to probe conformational flexibility of - Use of cysteine-reactive cross-linkers to probe conformational flexibility of
Prahlad J, Hauser DN, Milkovic NM, Cookson MR, Wilson MA. J Neurochem. 2014 Sep;130(6):839-53.
Development of a capillary isoelectric focusing immunoassay to measure DJ-1 - Development of a capillary isoelectric focusing immunoassay to measure DJ-1
Besong Agbo D, Klafki H, Poschmann G, Seyfarth K, Genius J, Janssen C, Stuhler K, Wurst W, Meyer HE, Klingenspor M, Wiltfang J. Anal Biochem. 2013 Dec 15;443(2):197-204.
Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat - Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat
O'Connell K, Ohlendieck K. Proteomics. 2009 Dec;9(24):5509-24.