PARK7

Gene Symbol PARK7
Entrez Gene 11315
Alt Symbol DJ-1, DJ1, HEL-S-67p
Species Human
Gene Type protein-coding
Description parkinson protein 7
Other Description Parkinson disease (autosomal recessive, early onset) 7|epididymis secretory sperm binding protein Li 67p|oncogene DJ1|protein DJ-1|protein deglycase DJ-1
Swissprots Q7LFU2 B2R4Z1 Q99497 Q6DR95 O14805
Accessions AAT68961 EAW71591 Q99497 AB073864 BAB71782 AF021819 AAC12806 AK091679 AK130886 AK312000 BAG34938 BC008188 AAH08188 BP248330 D61380 BAA09603 EU794641 ACJ13695 HQ447919 ADQ32403 XM_005263424 XP_005263481 NM_001123377 NP_001116849 NM_007262 NP_009193
Function Protein deglycase that repairs methylglyoxal- and glyoxal-glycated amino acids and proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteines, arginines and lysines residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) (PubMed:25416785). Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function (PubMed:17015834, PubMed:20304780, PubMed:18711745, PubMed:12796482, PubMed:19229105, PubMed:25416785). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for
Subcellular Location Cell membrane {ECO:0000250|UniProtKB:O88767}; Lipid-anchor {ECO:0000250|UniProtKB:O88767}. Cytoplasm {ECO:0000269|PubMed:12851414, ECO:0000269|PubMed:14579415, ECO:0000269|PubMed:15976810, ECO:0000269|PubMed:19229105}. Nucleus {ECO:0000269|PubMed:12851414, ECO:0000269|PubMed:14579415, ECO:0000269|PubMed:15976810}. Membrane raft {ECO:0000250|UniProtKB:O88767}. Mitochondrion {ECO:0000269|PubMed:15181200, ECO:0000269|PubMed:18711745, ECO:0000269|PubMed:19229105}. Note=Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage (PubMed:18711745). Detected in tau inclusions in brains from neurodegenerative disease patients (PubMed:14705119). Membrane raft localization in astrocytes and neuronal cells requires palmitoylation. {ECO:0000269|PubMed:14705119, ECO:0000
Tissue Specificity Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level). Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. {ECO:0000269|PubMed:14579415, ECO:0000269|PubMed:14662519, ECO:0000269|PubMed:14705119, ECO:0000269|PubMed:9070310}.

DJ-1 (D29E5) XP ® Rabbit mAb - 5933 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D29E5
Western blot analysis of extracts from MEF wild-type, MEF DJ-1 (-/-), HeLa, and C6 cells using DJ-1 (D29E5) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

DJ-1 (D21E11) Rabbit mAb - 5560 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D21E11
Western blot analysis of extracts from various cell lines and tissue using DJ-1 (D21E11) Rabbit mAb.

DJ-1 Antibody - 2134 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and CAD cells, using DJ-1 Antibody.

DJ-1 (C-16) - sc-27006 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DJ-1 (C-16): sc-27006. Western blot analysis of DJ-1 expression in HeLa whole cell lysate.

DJ-1 (N-20) - sc-27004 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DJ-1 (N-20): sc-27004. Western blot analysis of DJ-1 expression in HeLa whole cell lysate.

DJ-1 (FL-189) - sc-32874 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DJ-1 (FL-189): sc-32874. Western blot analysis of DJ-1 expression in HeLa whole cell lysate.

DJ-1 (mαDJ-1/E2-29) - sc-33665 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DJ-1 (mαDJ-1/E2-29): sc-33665. Western blot analysis of DJ-1 expression in HeLa whole cell lysate.

DJ-1 (D-4) - sc-55572 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DJ-1 (D-4): sc-55572. Western blot analysis of DJ-1 expression in HeLa whole cell lysate.

DJ-1 (A-9) - sc-55573 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DJ-1 (A-9): sc-55573. Western blot analysis of DJ-1 expression in HeLa whole cell lysate.

Anti-PARK7/DJ1 antibody - ab4150 from Abcam

Prices $392.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-PARK7/DJ1 antibody (ab4150) at 0.001 µg/mlLane 1 : HeLa cell lysate (in RIPA buffer)Lane 2 : Jurkat cell lysate (in RIPA buffer)Lysates/proteins at 35 µg per lane.developed using the ECL technique

Anti-PARK7/DJ1 antibody - ab53058 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PARK7/DJ1 antibody (ab53058) at 1/500 dilutionLane 1 : Extracts from HuvEc cellsLane 2 : Extracts from HuvEc cells with peptide

Anti-PARK7/DJ1 antibody - ab112015 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab112015, at 3.75µg/ml, staining PARK7/DJ1 in formalin-fixed, paraffin-embedded Human Prostate tissue by Immunohistochemistry.

Anti-PARK7/DJ1 antibody - ab74268 from Abcam

Prices $376.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab74268, at 1/50 dilution, staining DJ1 in formalin fixed, paraffin embedded human breast tissue by Immunohistochemistry.

Anti-PARK7/DJ1 antibody [E2.19] - ab130284 from Abcam

Prices $378.00
Sizes 125 µl
Host Mouse
Clonality Monoclonal
Clone E2.19
ab130284 at 1/1000 dilution, staining PARK7/DJ1 in Paraffin-embedded Human breast tissue by Immunohistochemistry, Followed by DAB detection after immunoreaction with a peroxidase conjugated anti-mouse IgG.

Anti-PARK7/DJ1 antibody [EP2815Y] - ab76008 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2815Y
Overlay histogram showing HepG2 cells stained with ab76008 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76008, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PARK7/DJ1 antibody [EP2816Y] - ab76241 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2816Y
Overlay histogram showing Jurkat cells stained with ab76241 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76241, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] - ab11251 from Abcam

Prices $398.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone malphaDJ-1/E2.19
Western blot using clone malphaDJ-1/E2.19 and a beta actin antibody as a loading control.The bottom band is PARK7/DJ1, the top band is beta actin.Lane 1: 293 cell lysateLane 2: MCF-7 cell lysateLanes 3-7: various different prostate cell linesLane 8: recombinant PARK7/DJ1 (that was used as immunogen for this antibody)

Anti-PARK7/DJ1 antibody [MJF-R16 (66-5)] - ab169520 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJF-R16 (66-5)
All lanes : Anti-PARK7/DJ1 antibody [MJF-R16 (66-5)] (ab169520) at 1/1000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate, treated with hydrogen peroxideLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-PARK7/DJ1 antibody, prediluted - ab74317 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Staining of formalin-fixed, paraffin-embedded tissue human breast tissue with ab74317.

Anti-PARK7 / DJ-1 Antibody - LS-C14859 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-PARK7 / DJ-1 Antibody (N-Terminus) - LS-C14860 from LifeSpan Bioscience

Prices $485.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal

Anti-PARK7 / DJ-1 Antibody - LS-C38081 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal

Anti-PARK7 / DJ-1 Antibody (C-Terminus) - LS-C64733 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-PARK7 / DJ-1 Antibody (C-Terminus) - LS-C50765 from LifeSpan Bioscience

Prices $530.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
Human breast stained with Anti-DJ-1

Anti-PARK7 / DJ-1 Antibody - LS-C83396 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Search more