Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19]
| Name | Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] |
|---|---|
| Supplier | Abcam |
| Catalog | ab11251 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | malphaDJ-1/E2.19 |
| Applications | FC IHC-F ICC/IF ICC/IF WB ICC/IF IHC-F |
| Species Reactivities | Human, Zebrafish |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | PARK7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot using clone malphaDJ-1/E2.19 and a beta actin antibody as a loading control.The bottom band is PARK7/DJ1, the top band is beta actin.Lane 1: 293 cell lysateLane 2: MCF-7 cell lysateLanes 3-7: various different prostate cell linesLane 8: recombinant PARK7/DJ1 (that was used as immunogen for this antibody)
Western blot using ab11251 at 1/500 on HeLa whole cell lysate (20µg/lane).
ICC/IF image of ab11251 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab11251, 1:500 dilution) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
![Overlay histogram showing HepG2 cells stained with ab11251 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11251, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7316_PARK7DJ1-Primary-antibodies-ab11251-3.jpg)
Overlay histogram showing HepG2 cells stained with ab11251 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11251, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Identification of prostate-enriched proteins by in-depth proteomic analyses of - Identification of prostate-enriched proteins by in-depth proteomic analyses of
Principe S, Kim Y, Fontana S, Ignatchenko V, Nyalwidhe JO, Lance RS, Troyer DA, Alessandro R, Semmes OJ, Kislinger T, Drake RR, Medin JA. J Proteome Res. 2012 Apr 6;11(4):2386-96.
Identification of differentially expressed proteins in direct expressed prostatic - Identification of differentially expressed proteins in direct expressed prostatic
Kim Y, Ignatchenko V, Yao CQ, Kalatskaya I, Nyalwidhe JO, Lance RS, Gramolini AO, Troyer DA, Stein LD, Boutros PC, Medin JA, Semmes OJ, Drake RR, Kislinger T. Mol Cell Proteomics. 2012 Dec;11(12):1870-84.
The cytotoxic pathway triggered by palytoxin involves a change in the cellular - The cytotoxic pathway triggered by palytoxin involves a change in the cellular
Sala GL, Bellocci M, Rossini GP. Chem Res Toxicol. 2009 Dec;22(12):2009-16.
Protein profiling of microdissected pancreas carcinoma and identification of - Protein profiling of microdissected pancreas carcinoma and identification of
Melle C, Ernst G, Escher N, Hartmann D, Schimmel B, Bleul A, Thieme H, Kaufmann R, Felix K, Friess HM, Settmacher U, Hommann M, Richter KK, Daffner W, Taubig H, Manger T, Claussen U, von Eggeling F. Clin Chem. 2007 Apr;53(4):629-35. Epub 2007 Feb 15.
p53-dependent neuronal cell death in a DJ-1-deficient zebrafish model of - p53-dependent neuronal cell death in a DJ-1-deficient zebrafish model of
Bretaud S, Allen C, Ingham PW, Bandmann O. J Neurochem. 2007 Mar;100(6):1626-35. Epub 2006 Dec 12.
Association of PINK1 and DJ-1 confers digenic inheritance of early-onset - Association of PINK1 and DJ-1 confers digenic inheritance of early-onset
Tang B, Xiong H, Sun P, Zhang Y, Wang D, Hu Z, Zhu Z, Ma H, Pan Q, Xia JH, Xia K, Zhang Z. Hum Mol Genet. 2006 Jun 1;15(11):1816-25. Epub 2006 Apr 21.
Differential control of apoptosis by DJ-1 in prostate benign and cancer cells. - Differential control of apoptosis by DJ-1 in prostate benign and cancer cells.
Hod Y. J Cell Biochem. 2004 Aug 15;92(6):1221-33.