All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : Hela cell lysateLane 3 : HepG2 cell lysateLane 4 : MDA-MB-435 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/1000 dilutionLane 1 : PC-12 cell lysateLane 2 : NIH-3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of formalin fixed paraffin embedded Human papillary adenocarcinoma of thyroid labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG. Counterstained with Haematoxylin.
Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG. Counterstained with Haematoxylin.
Immunohistochemical analysis of Hela cells fixed in 4% paraformaldehyde labeling PARN with ab188333 at 1/100 and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Counterstained with DAPI (blue).
Flow cytometric analysis of Hela cells fixed in 2% paraformaldehyde labeling PARN with ab188333 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.