PARN

Gene Symbol PARN
Entrez Gene 5073
Alt Symbol DAN
Species Human
Gene Type protein-coding
Description poly(A)-specific ribonuclease
Other Description deadenylating nuclease|deadenylation nuclease|poly(A)-specific ribonuclease (deadenylation nuclease)|poly(A)-specific ribonuclease PARN|polyadenylate-specific ribonuclease
Swissprots B4DWR4 O95453 B4E1H6 B4DDG8 B4DSB0 B2RCB3
Accessions EAW85110 EAW85111 O95453 AJ005698 CAA06683 AK023866 BAG51231 AK293189 BAG56729 AK299653 BAG61572 AK301648 BAG63126 AK303348 BAG64410 AK315020 BAG37510 AW338742 BC044763 BC050029 AAH50029 BF435512 DA308295 JF432357 ADZ15574 XM_011522510 XP_011520812 XM_011522511 XP_011520813 XM_011522512 XP_011520814 XM_011522513 XP_011520815 XM_011522514 XP_011520816 XM_011546699 XP_011545001 XM_011546700 XP_011545002 XM_011546701 XP_011545003 XM_011546702 XP_011545004 XM_011546703 XP_011545005 NM_001134477 NP_001127949 NM_001242992 NP_001229921 NM_002582 NP_002573
Function 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. {ECO:0000269|PubMed:10882133, ECO:0000269|PubMed:11359775, ECO:0
Subcellular Location Nucleus. Cytoplasm. Nucleus, nucleolus. Note=Some nuclear fraction is nucleolar.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:10640832, ECO:0000269|PubMed:9736620}.
Top Pathways RNA degradation

PARN (A42) Antibody - 3894 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using PARN (A42) Antibody.

PARN (P620) Antibody - 3899 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using PARN (P620) Antibody.

PARN (H-105) - sc-135242 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PARN (H-105): sc-135242. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing cytoplasmic and nuclear staining of glandular cells.

PARN (N-12) - sc-47618 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PARN (N-20) - sc-47619 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PARN antibody - ab89831 from Abcam

Host Mouse
Clonality Polyclonal
Anti-PARN antibody (ab89831) at 1 µg/ml + PC12 cell lysate at 50 µgdeveloped using the ECL technique

Anti-PARN antibody - ab27778 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PARN antibody (ab27778) at 1 µg/mlLane 1 : HeLa Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Anti-PARN antibody - ab125185 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PARN antibody (ab125185) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-PARN antibody [4D6] - N-terminal - ab180404 from Abcam

Prices $357.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D6
All lanes : Anti-PARN antibody [4D6] - N-terminal (ab180404) at 1/2000 dilutionLane 1 : Non-transfected HEK293T cell lysateLane 2 : PARN transfected HEK293T cell lysateLysates/proteins at 5 µg per lane.

Anti-PARN antibody [EPR11670(2)] - ab188333 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11670(2)
All lanes : Anti-PARN antibody [EPR11670(2)] (ab188333) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : Hela cell lysateLane 3 : HepG2 cell lysateLane 4 : MDA-MB-435 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PARN Antibody (aa1-266, clone 4D12) - LS-C336625 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D12
IHC of paraffin-embedded Human lung tissue using anti-PARN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-PARN Antibody (clone 13A213) - LS-C183404 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 13A213

Anti-PARN Antibody (aa539-589) - LS-C290227 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PARN Antibody (aa589-639) - LS-C290230 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PARN Antibody (FITC) - LS-C318432 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PARN Antibody (HRP) - LS-C318436 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PARN Antibody (N-Terminus) - LS-C31344 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
SP2/0 Cell Lysate

Anti-PARN Antibody - LS-C332970 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of MDA-MB-453s cell lines, using PARN antibody.

Anti-PARN Antibody (aa1-266, clone 4D6) - LS-C336601 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN.

Anti-PARN Antibody (aa1-266, clone 2D3) - LS-C336616 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN.

Anti-PARN Antibody (aa1-266, clone 4E12) - LS-C336615 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4E12
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN.

Anti-PARN Antibody (aa1-266, clone 3G9) - LS-C336617 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3G9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN.

Anti-PARN Antibody (aa1-266, clone 4C9) - LS-C336618 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN.

Anti-PARN Antibody (aa1-266, clone 2D9) - LS-C336648 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN.

Anti-PARN Antibody (aa1-266, clone 1G7) - LS-C336647 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G7
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN.
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