Anti-PATJ antibody
| Name | Anti-PATJ antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab102113 |
| Prices | $385.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 60-287 of Human PATJ (AAI42662) |
| Description | Rabbit Polyclonal |
| Gene | INADL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab102113 stained CaC02 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102113 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-PATJ antibody (ab102113) at 1/5000 dilution + A431 whole cell lysate at 30 µg
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ab102113 at 1/100 dilution.