All lanes : Anti-PCBP2/hnRNP E2 antibody [376CT13.1.2] (ab61334) at 5 µg/mlLane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutionPerformed under reducing conditions.