All lanes : Anti-PCGF1 antibody [EPR13037(2)] (ab183499) at 1/20000 dilutionLane 1 : Human fetal brain lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling PCGF1 with ab183499 at 1/100 dilution, compared to a Rabbit monoclonal IgG isotype control, followed by a Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.